NEB, M1524L, TET2
Catalog Number: M1524L
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Pack Size1 unit / pack
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[{"group": "General", "name": "Materials Required but not Supplied Monarch PCR & DNA Cleanup Kit (NEB #T1130), or Thermolabile Proteinase K (NEB #P8111) Nuclease-free Water (NEB #B1500) Unit Definition One unit is defined as the amount of enzyme required to modify 1.4 pmol of a 21-mer fluorescently labeled oligonucleotide duplex containing a central methylated CpG moiety in a total reaction volume of 20 μl in 1 hour at 37°C. Reaction Conditions 1X TET2 Reaction Buffer Incubate at 37°C Heat Inactivation No Unit Assay Conditions A fluorescently labeled dsDNA oligonucleotide containing a single 5mC is incubated with 1 unit of TET2 in 1X TET2 Reaction Buffer (containing supplement and Fe2+). 1 unit of TET2 must modify ≥80% of 1.75 pmol of substrate as determined by capillary electrophoresis.", "value": ""}]
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