NEB, M2081L, Faustovirus Capping Enzyme
Catalog Number: M2081L
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Category
Pack Size2081 L
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[{"group": "General", "name": "Unit Definition One unit of Faustovirus Capping Enzyme is defined as the amount of enzyme required to convert 75 pmol of a 20-mer ppp-RNA to Cap-0 RNA in 30 minutes at 37°C. Reaction Conditions 1X Capping Buffer Supplement with 0.5 mM GTP and 0.1 mM S-adenosylmethionine (SAM) Incubate at 37°C 1X Capping Buffer 50 mM Tris-HCl 5 mM KCl 1 mM MgCl2 1 mM DTT (pH 8 @ 25°C) Heat Inactivation 70°C for 10 minutes Addition of EDTA to 5 mM is recommended to avoid RNA hydrolysis.", "value": ""}]
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