NEB, M2622L, Hi-T4™ DNA Ligase
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Catalog Number: M2622L
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Category
Pack Size2622 L
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to give 50% ligation of 6 µg of Lambda-HindIII DNA in 30 minutes at 25°C in a total reaction volume of 20 μl. Reaction Conditions 1X T4 DNA Ligase Reaction Buffer Incubate at 25°C 1X T4 DNA Ligase Reaction Buffer 50 mM Tris-HCl 10 mM MgCl2 1 mM ATP 10 mM DTT (pH 7.5 @ 25°C) Storage Buffer 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7.4 @ 25°C Heat Inactivation 65°C for 10 minutes", "value": ""}]
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