NEB, M5508L, Thermolabile USER® II Enzyme

[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to nick 10 pmol of a fluorescently labeled 34 mer oligonucleotide duplex containing a single uracil base in 15 minutes at 37°C in a total reaction volume of 10 μl in 1X T4 DNA Ligase Buffer. Reaction Conditions 1X rCutSmart™ Buffer Incubate at 37°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) Activity in NEBuffers Storage Buffer 15 mM Tris-HCl 35 mM NaCl 25 mM KCl 1 mM DTT 0.1 mM EDTA 100 µg/ml BSA 50% Glycerol pH 7.5 @ 25°C Heat Inactivation 65°C for 10 minutes", "value": ""}]