[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to nick 10 pmol of a fluorescently labeled 60 mer oligonucleotide duplex containing a single uracil base, in 15 minutes at 65°C in a total reaction volume of 10 μl in 1X Thermopol Buffer. Reaction Conditions 1X ThermoPol® Reaction Buffer Incubate at 65°C 1X ThermoPol® Reaction Buffer 20 mM Tris-HCl 10 mM (NH4)2SO4 10 mM KCl 2 mM MgSO4 0.1% Triton® X-100 (pH 8.8 @ 25°C) Storage Buffer 15 mM Tris-HCl 35 mM NaCl 1 mM DTT 0.1 mM 100 µg/ml BSA 50% Glycerol pH 7.4 @ 25°C", "value": ""}]
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