| <b>Materials Required but not Supplied</b> 1X TE (10 mM Tris pH 8.0, 1 mM EDTA) 0.2 ml thin wall PCR tubes Magnetic rack/stand (NEB #S1515S; Alpaqua® #A001322 or equivalent) PCR machine Vortex Microcentrifuge Bioanalyzer®, TapeStation® or other fragment analyzer and associated consumables 80% Ethanol For use with NEBNext UltraShear Protocol | SPRIselect™ Reagent Kit (Beckman Coulter, Inc. #B23317), AMPure® XP Beads (Beckman Coulter, Inc. #A63881) or Monarch®® PCR & DNA Cleanup Kit (NEB# T1030S/L) are recommended for Section 1. For use with NEBNext Ultra II End Repair/dA-Tailing Module Protocol: NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546S/L) for Section 2. Recommended Material Not Included: NEBNext Ultra II Ligation Module (NEB #E7595) and NEBNext Multiplex Oligos (www.neb.com/oligos). For use with NEBNext Enzymatic Methyl-seq Protocol: NEBNext Enzymatic Methyl-seq Kit (NEB #E7120S/L) for Section 3. Formamide (Sigma #F9037-100 ml) or optional 0.1 N NaOH. Formamide is preferred. If using NaOH, please see NEBNext Enzymatic Methyl-seq Kit (NEB #E7120) FAQs Nuclease-free |
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