NEB, M7635L, Duplex DNase
Catalog Number: M7635L
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Pack Size000 units / pack
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to release 3 pmol of FAM from a 35-mer FAM-BHQ1 labeled hairpin oligonucleotide in 1 minute at 30°C in a 50 μl reaction volume. Reaction Conditions 1X NEBuffer™ r2.1 1X NEBuffer™ r2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) Usage Concentration 2,000 units/ml Storage Buffer 10 mM Tris-HCl 50 mM KCl 50% Glycerol pH 7.4 @ 25°C Heat Inactivation 75°C for 10 minutes in the presence of 1 mM DTT. If the sample contains RNA, we recommend adding EDTA (10 mM final concentration) and DTT (1 mM final concentration), prior to heat inactivation. RNA may degrade at temperatures > 65°C in the presence of divalent metals such as Mg2+.", "value": ""}]
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