[{"group": "General", "name": "Materials Required but not Supplied Nuclease-free Water (NEB #B1500) Unit Definition One unit is defined as the amount of enzyme required to convert 0.5 µg relaxed pUC19 plasmid to the supercoiled form in 1X Gyrase Reaction Buffer incubated for 15 minutes at 37°C in a total reaction volume of 30 μl. Reaction Conditions 1X Gyrase Reaction Buffer Incubate at 37°C Heat Inactivation 65°C for 20 minutes", "value": ""}]
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