NEB, P0706S, Remove-iT® PNGase F
Remove-iT
Catalog Number: P0706S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 5 μg of DTT denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 μl. <b>Reaction Conditions</b> 1X GlycoBuffer 2 Incubate at 37°C 1X GlycoBuffer 2 50 mM Sodium Phosphate (pH 7.5 @ 25°C) <b>Storage Buffer</b> 20 mM Tris-HCl 50 mM NaCl 5 mM EDTA pH 7.5 @ 25°C <b>Heat Inactivation</b> 75°C for 10 minutes <b>Molecular Weight</b> Apparent | 41 kDa <b>Unit Assay Conditions</b> 5 µg of RNase B are denatured with 1X DTT at 55°C for 10 minutes. After the addition of 1X GlycoBuffer 2, two-fold dilutions of Remove-iT PNGase F are added and the reaction mix is incubated for 1 hour at 37°C. Separation of reaction products is visualized by SDS-PAGE. |
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