NEB, P0709L, PNGase F (Glycerol-free), Recombinant
PNGase F is the most effective enzymatic method for removing almost all
Catalog Number: P0709L
Send an RFQ.
Category
Pack Size0709 L
TagsProtein Tools
Loading…
| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 μg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 μl. 1X Glycoprotein Denaturing Buffer 0.5% SDS 40 mM DTT 1X NP-40 1% NP-40 in MilliQ-H 2 O <b>Reaction Conditions</b> 1X GlycoBuffer 2 Incubate at 37°C 1X GlycoBuffer 2 50 mM Sodium Phosphate (pH 7.5 @ 25°C) <b>Storage Buffer</b> 50 mM NaCl 20 mM Tris-HCl 5 mM EDTA pH 7.5 @ 25°C <b>Heat Inactivation</b> 75°C for 10 minutes <b>Molecular Weight</b> Apparent | 36000 daltons <b>Unit Assay Conditions</b> 10 μg of RNase B are denatured with 1X Glycoprotein Denaturing Buffer at 100°C for 10 minutes. After the addition of NP-40 and GlycoBuffer 2, two-fold dilutions of PNGase F (Glycerol-free), Recombinant are added and the reaction mix is incubated for 1 hour at 37°C. Separation of reaction products are visualized by SDS-PAGE. |
|---|
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
