| <b>Unit Definition</b> One unit of enzyme cleaves 1 pmol of Neuraminidase treated FAM-labelled O-glycopeptide in 1 hour at 37 °C. <b>Reaction Conditions</b> 1X GlycoBuffer 2 Incubate at 37°C 1X GlycoBuffer 2 50 mM Sodium Phosphate (pH 7.5 @ 25°C) <b>Storage Buffer</b> 20 mM Tris-HCl 50 mM NaCl 1 mM EDTA pH 7.5 @ 25°C <b>Heat Inactivation</b> 65°C for 10 minutes <b>Molecular Weight</b> Apparent | 147 kDa <b>Unit Assay Conditions</b> Two fold serial dilutions of O-Glycosidase are added to reaction mixtures of 2 pmol Neuraminidase treated FAM-labelled O-glycopeptide in 1X GlycoBuffer 2. The reactions are incubated at 37 °C for 1 hour. The amount of deglycosylated O-glycopeptide is determined by capillary electrophoresis. |
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