| <b>Unit Definition</b> One unit is defined as the amount of enzyme that will liberate 0.25 nmol unsaturated oligosaccharides from porcine mucosal heparin per minute at 30°C and pH 7.0 in a total reaction volume of 100 μl. <b>Reaction Conditions</b> 1X Bacteroides Heparinase Reaction Buffer Incubate at 30°C <b>Storage Buffer</b> 20 mM Tris-HCl 100 mM NaCl 5 mM CaCl2 1 mM EDTA pH 7.5 @ 25°C <b>Heat Inactivation</b> 100°C for 1 minute <b>Molecular Weight</b> Apparent | 42 kDa <b>Unit Assay Conditions</b> Two fold dilutions of Bacteroides Heparinase I are incubated with 1 mg/ml porcine mucosal heparin substrate in 1X Bacteroides Heparinase Reaction Buffer, in a 100 μl reaction. The reaction mix is incubated at 30°C. Liberation of unsaturated oligosaccharides is detected by real-time UV spectroscopy at 232 nm. |
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