NEB, P0741S, Endo S
Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG
Catalog Number: P0741S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 5 μg of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10 μl. <b>Reaction Conditions</b> 1X GlycoBuffer 1 Incubate at 37°C 1X GlycoBuffer 1 5 mM CaCl2 50 mM sodium acetate (pH 5.5 @ 25°C) <b>Storage Buffer</b> 20 mM Tris-HCl 50 mM NaCl 5 mM EDTA pH 7.5 @ 25°C <b>Heat Inactivation</b> 55°C for 10 minutes <b>Molecular Weight</b> Apparent | 136 kDa <b>Unit Assay Conditions</b> 5 µg of IgG in 1X GlycoBuffer 1 are incubated with two-fold dilutions of Endo S for 1 hour at 37°C. Separation of reaction products is visualized by SDS-PAGE. |
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