NEB, P0753L, Lambda Protein Phosphatase (Lambda PP)
Lambda Protein Phosphatase is a Mn
Catalog Number: P0753L
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Category
Pack Size000 units / pack
TagsProtein Tools
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p -Nitrophenyl Phosphate (50 mM) (NEB #P0757) in 1 minute at 30°C in a total reaction volume of 50 μl. <b>Reaction Conditions</b> 1X NEBuffer Pack for Protein MetalloPhosphatases (PMP) Supplement with 1 mM MnCl2 Incubate at 30°C 1X NEBuffer Pack for Protein MetalloPhosphatases (PMP) 50 mM HEPES 100 mM NaCl 2 mM DTT 0.01% Brij 35 (pH 7.5 @ 25°C) <b>Storage Buffer</b> 50 mM HEPES 100 mM NaCl 2 mM DTT 0.01% Brij 35 0.1 mM EGTA 0.1 mM MnCl2 50% Glycerol pH 7.5 @ 25°C <b>Heat Inactivation</b> 65°C for 60 minutes <b>Molecular Weight</b> Theoretical | 25 kDa <b>Specific Activity</b> 800,000 units/mg <b>Storage Notes</b> Avoid repeated freeze/thaw cycles. <b>Shipping Notes</b> Ships on dry ice |
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