NEB, P0768S, α1-2,3,6 Mannosidase
Catalog Number: P0768S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to cleave > 95% of the terminal mannose from 1 nmol of Man(α1,3)-Man(β1,4)-GlcNAc-7-amino-4-methyl-coumarin (AMC), in 1 hour at 37°C in a total reaction volume of 10 μl. <b>Reaction Conditions</b> 1X GlycoBuffer 4 Supplement with 1X Zinc Incubate at 37°C 1X GlycoBuffer 4 50 mM sodium acetate (pH 4.5 @ 25°C) <b>Storage Buffer</b> 20 mM Tris-HCl 50 mM NaCl pH 7.5 @ 25°C <b>Heat Inactivation</b> 95°C for 10 minutes <b>Molecular Weight</b> Theoretical | 110 kDa <b>Unit Assay Conditions</b> Two fold dilutions of α1-2,3,6 Mannosidase are incubated with 1 nmol AMC-labeled substrate, 1X GlycoBuffer 4 and 1X Zinc in a 10 μl reaction. The reaction mix is incubated at 37°C for 1 hour. Separation of reaction products are visualized via thin layer chromatography (1). |
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