| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to cleave > 95% of 1 µg of human IgG, in 15 minutes at 37°C in a total reaction volume of 10 μl. <b>Reaction Conditions</b> 1X GlycoBuffer 2 Incubate at 37°C 1X GlycoBuffer 2 50 mM Sodium Phosphate (pH 7.5 @ 25°C) <b>Storage Buffer</b> 20 mM Tris-HCl 50 mM NaCl 1 mM EDTA pH 7.5 @ 25°C <b>Heat Inactivation</b> 65°C for 10 minutes <b>Molecular Weight</b> Theoretical | 35578 kDa <b>Unit Assay Conditions</b> Two fold dilutions of IdeZ Protease (IgG-specific) are incubated with 1 μg of human IgG and 1X GlycoBuffer 2 in a 10 μl reaction. The reaction mix is incubated for 15 minutes at 37°C. Separation of reaction products are visualized by SDS-PAGE. <b>Storage Notes</b> Avoid repeated freeze/thaw cycles. |
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