NEB, P0777S, Endo-β-Galactosidase
Catalog Number: P0777S
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Category
TagsGlycobiology
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| <b>Unit Definition</b> The enzyme activity is determined by its ability to cleave over 95% of 2 nmol Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc-7-amino-4-methyl-coumarin (AMC) using less than 10 ng of enzyme within 1 hour at 37°C, in a total reaction volume of 20 μl. <b>Reaction Conditions</b> 1X GlycoBuffer 3 Incubate at 37°C 1X GlycoBuffer 3 50 mM sodium acetate (pH 6 @ 25°C) <b>Storage Buffer</b> 50 mM sodium acetate 50 mM NaCl pH 5.5 @ 25°C <b>Heat Inactivation</b> 55°C for 10 minutes <b>Molecular Weight</b> Apparent | 40.8 kDa <b>Unit Assay Conditions</b> Two fold dilutions of Endo-β-Galactosidase are incubated with 2 nmol AMC labeled substrate in 1X GlycoBuffer 3 in a 20 μl reaction. The reaction mix is incubated at 37°C for 1 hour. Separation of reaction products are visualized via thin layer chromatography. |
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