NEB, P6000S, cAMP-dependent Protein Kinase (PKA), catalytic subunit
Catalog Number: P6000S
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| <b>Unit Definition</b> One unit is defined as the amount of PKA catalytic subunit required to catalyze the transfer of 1 pmol of phosphate to Kemptide, LRRA S LG (100 µM) in 1 minute at 30°C in a total reaction volume of 25μL. <b>Reaction Conditions</b> 1X NEBuffer™ for Protein Kinases (PK) Supplement with 200 µM ATP Incubate at 30°C 1X NEBuffer™ for Protein Kinases (PK) 50 mM Tris-HCl 10 mM MgCl2 0.1 mM EGTA 2 mM DTT 0.01% Brij 35 (pH 7.5 @ 25°C) <b>Storage Buffer</b> 20 mM Tris-HCl 50 mM NaCl 2 mM DTT 1 mM EDTA 50% Glycerol pH 7.5 @ 25°C <b>Heat Inactivation</b> 65°C for 20 minutes <b>Molecular Weight</b> Theoretical | 38 kDa |
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