[{"group": "General", "name": "Unit Definition One unit of enzyme catalyzes the phosphorylation of 1 pmol of RRRDDD S DDD substrate in 10 minutes at 30˚C. Reaction Conditions 1X NEBuffer™ for Protein Kinases (PK) Supplement with 200 µM ATP Incubate at 30°C 1X NEBuffer™ for Protein Kinases (PK) 50 mM Tris-HCl 10 mM MgCl2 0.1 mM EGTA 2 mM DTT 0.01% Brij 35 (pH 7.5 @ 25°C) Storage Buffer 20 mM Tris-HCl 350 mM NaCl 1 mM EDTA 2 mM DTT 0.1% Triton® X-100 pH 7.5 @ 25°C Storage Notes Avoid repeated freeze/thaw cycles.", "value": ""}]
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