| <b>Unit Definition</b> 1 μg of Factor Xa will cleave 50 μg of MBP-fusion protein test substrate, MBP-FXa-Paramyosin-ΔSal to 95% completion in a total reaction volume of 50 μl in 6 hours or less at 25°C in 20 mM Tris-HCl (pH 8.0 @ 25°C) with 100 mM NaCl and 2 mM CaCl2. <b>Usage Concentration</b> 1 mg/ml <b>Removal</b> Factor Xa will bind specifically to benzamidine-agarose. <b>Storage Buffer</b> 50 mM HEPES 500 mM NaCl 2 mM CaCl2 50% Glycerol pH 8 @ 25°C <b>Molecular Weight</b> Theoretical | 43 kDa <b>Deactivation</b> Dansyl-glu-gly-arg-chloromethyl ketone (CALBIOCHEM, #251700) will irreversibly inactivate Factor Xa by covalent attachment at the active site. In a reaction containing 20 μg/ml Factor Xa, 2 μM dansyl-glu-gly-arg-chloromethyl ketone will inactivate >95% of the Factor Xa in 1 minute at room temperature. |
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