NEB, P8070S, Enterokinase, light chain
Catalog Number: P8070S
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Category
Pack Size1 unit / pack
TagsProtein Tools
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| <b>Unit Definition</b> 1 unit is defined as the amount of enzyme required to cleave 25 µg of a MBP-EK-paramyosin-ΔSal substrate to 95% completion in 16 hours at 25°C in a total reaction volume of 25 μl. <b>Removal</b> Enterokinase will bind specifically to trypsin inhibitor agarose. <b>Storage Buffer</b> 20 mM Tris-HCl 200 mM NaCl 2 mM CaCl2 50% Glycerol pH 7.2 @ 4°C <b>Molecular Weights</b> Apparent | 31 kDa Theoretical: 26.3 kDa <b>Unit Assay Conditions</b> Two fold dilutions of Enterokinase, light chain are incubated with 25 µg MBP-EK-paramyosin-ΔSal substrate in 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl 2 (pH 8.0 @ 25°C) in a 25 μl reaction. The reaction mix is incubated at 25°C for 16 hours. Separation of reaction products are visualized by SDS-PAGE. |
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