NEB, P8118S, Cold-Active TEV Protease
Catalog Number: P8118S
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| <b>Unit Definition</b> One unit of Cold-Active TEV Protease will cleave 2 µg of MBP-fusion protein, MBP5-TEV-paramyosin ΔSal, to 95% completion in a total reaction volume of 10 μl in 5 hours at 4°C in 50 mM Tris-HCl (pH 7.5 @ 25°C) with 0.5 mM EDTA and 1 mM DTT. Storage Temperature | -20°C . <b>Reaction Conditions</b> 1X TEV Protease Reaction Buffer Incubate at 4°C 1X TEV Protease Reaction Buffer 50 mM Tris-HCl 0.5 mM EDTA 1 mM DTT (pH 7.5 @ 25°C) <b>Storage Buffer</b> 50 mM Tris-HCl 250 mM NaCl 1 mM TCEP 1 mM EDTA 50% Glycerol pH 7.5 @ 25°C <b>Heat Inactivation</b> 65°C for 10 minutes <b>Molecular Weight</b> Apparent: 28 kDa |
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