NEB, R0101T, EcoRI
EcoRI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10227213.
Catalog Number: R0101T
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Category
Pack Size100 units / pack
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl. <b>Reaction Conditions</b> 1X NEBuffer™ EcoRI/SspI Incubate at 37°C 1X NEBuffer™ EcoRI/SspI 100 mM Tris-HCl 50 mM NaCl 10 mM MgCl2 0.025% Triton® X-100 (pH 7.5 @ 25°C) <b>Activity in NEBuffers</b> NEBuffer™ r1.1 | 25% NEBuffer™ r2.1: 100% NEBuffer™ r3.1: 50% rCutSmart™ Buffer: 50% <b>Diluent Compatibility</b> Diluent C <b>Storage Buffer</b> 10 mM KPO4 300 mM NaCl 0.1 mM EDTA 200 µg/ml Recombinant Albumin 50% Glycerol 1 mM DTT 0.15% Triton® X-100 pH 7 @ 25°C <b>Heat Inactivation</b> 65°C for 20 minutes <b>Methylation Sensitivity</b> dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by Some Combinations of Overlapping |
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