NEB, R0104T, HindIII
HindIII has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10152649.
Catalog Number: R0104T
Send an RFQ.
Category
Pack Size100 units / pack
Loading…
| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl. <b>Reaction Conditions</b> 1X NEBuffer™ r2.1 Incubate at 37°C 1X NEBuffer™ r2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Activity in NEBuffers</b> NEBuffer™ r1.1 | 25% NEBuffer™ r2.1: 100% NEBuffer™ r3.1: 50% rCutSmart™ Buffer: 50% <b>Diluent Compatibility</b> Diluent B <b>Storage Buffer</b> 300 mM NaCl 10 mM Tris-HCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 500 µg/ml Recombinant Albumin pH 7.4 @ 25°C <b>Heat Inactivation</b> 80°C for 20 minutes <b>Methylation Sensitivity</b> dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive |
|---|
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
