NEB, R0123S, SfiI
Requires two or more sites for cleavage. Please review the enzyme list and recommendations in the following
Catalog Number: R0123S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 50°C in a total reaction volume of 50 μl. <b>Reaction Conditions</b> 1X rCutSmart™ Buffer Incubate at 50°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Activity in NEBuffers</b> NEBuffer™ r1.1 | 25% NEBuffer™ r2.1: 100% NEBuffer™ r3.1: 50% rCutSmart™ Buffer: 100% <b>Diluent Compatibility</b> Diluent C <b>Storage Buffer</b> 10 mM Tris-HCl 250 mM NaCl 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol 0.15% Triton® X-100 1 mM DTT pH 7.4 @ 25°C <b>Heat Inactivation</b> No <b>Methylation Sensitivity</b> dam methylation: Not Sensitive dcm methylation: Impaired by Overlapping CpG Methylation: Blocked by Some Combinations of Overlapping <b>Activity at Temperature</b> @37°C: 10% |
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