NEB, R0544S, KasI
KasI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10211225.
Catalog Number: R0544S
Send an RFQ.
Category
Loading…
| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to digest 1 µg of pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 μl. <b>Reaction Conditions</b> 1X rCutSmart™ Buffer Incubate at 37°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Activity in NEBuffers</b> NEBuffer™ r1.1 | 50% NEBuffer™ r2.1: 100% NEBuffer™ r3.1: 50% rCutSmart™ Buffer: 100% <b>Diluent Compatibility</b> Diluent B <b>Storage Buffer</b> 100 mM KCl 10 mM Tris-HCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 200 µg/ml Recombinant Albumin pH 7 @ 25°C <b>Heat Inactivation</b> 65°C for 20 minutes <b>Methylation Sensitivity</b> dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked |
|---|
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
