NEB, R0588S, FseI
Catalog Number: R0588S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to digest 1 µg of pBC4 DNA in 1 hour at 37°C in a total reaction volume of 50 μl. <b>Reaction Conditions</b> 1X rCutSmart™ Buffer Incubate at 37°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Activity in NEBuffers</b> NEBuffer™ r1.1 | 100% NEBuffer™ r2.1: 75% NEBuffer™ r3.1: <10% rCutSmart™ Buffer: 100% <b>Diluent Compatibility</b> Diluent B <b>Storage Buffer</b> 10 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 0.5% Tween® 20 0.5% IGEPAL® CA-630 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> 65°C for 20 minutes <b>Methylation Sensitivity</b> dam methylation: Not Sensitive dcm methylation: Impaired by Some Combinations of Overlapping CpG Methylation: Blocked |
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