NEB, R0607L, Nt.BstNBI
Nt.BstNBI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10159526.
Catalog Number: R0607L
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Category
Pack Size0607 L
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to digest 1 µg T7 DNA in 1 hour at 55°C in a total reaction volume of 50 μl. The phage T7 DNA is a particularly convenient substrate for assaying the activity because of the proximity of GAGTC sequences. Nt.BstNBI cleavage of phage T7 DNA yields 5 bands; 13 kb, 9 kb, 7 kb, 6 kb and 4 kb. <b>Reaction Conditions</b> 1X NEBuffer™ r3.1 Incubate at 55°C 1X NEBuffer™ r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Activity in NEBuffers</b> NEBuffer™ r1.1 | 0% NEBuffer™ r2.1: 10% NEBuffer™ r3.1: 100% rCutSmart™ Buffer: 10% <b>Diluent Compatibility</b> Diluent A <b>Storage Buffer</b> 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 200 µg/ml Recombinant Albumin 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> 80°C for 20 minutes <b>Methylation Sensitivity</b> dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive <b>Activity at Temperature</b> @37°C: 50% |
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