| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl. <b>Reaction Conditions</b> 1X rCutSmart™ Buffer Incubate at 37°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Activity in NEBuffers</b> NEBuffer™ r1.1 | 100% NEBuffer™ r2.1: 100% NEBuffer™ r3.1: 25% rCutSmart™ Buffer: 100% <b>Storage Buffer</b> 10 mM Tris-HCl 300 mM NaCl 0.1 mM EDTA 200 µg/ml Recombinant Albumin 50% Glycerol 0.5 mM NiSO4 pH 7.4 @ 25°C <b>Heat Inactivation</b> 65°C for 20 minutes <b>Methylation Sensitivity</b> dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Impaired by Overlapping |
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