NEB, R0647S, BtsCI
Catalog Number: R0647S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 50°C in a total reaction volume of 50 μl. <b>Reaction Conditions</b> 1X rCutSmart™ Buffer Incubate at 50°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Activity in NEBuffers</b> NEBuffer™ r1.1 | 10% NEBuffer™ r2.1: 100% NEBuffer™ r3.1: 25% rCutSmart™ Buffer: 100% <b>Diluent Compatibility</b> Diluent B <b>Storage Buffer</b> 10 mM Tris-HCl 300 mM NaCl 0.1 mM EDTA 500 µg/ml BSA 50% Glycerol 1 mM DTT pH 7.4 @ 25°C <b>Heat Inactivation</b> 80°C for 20 minutes <b>Methylation Sensitivity</b> dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive <b>Activity at Temperature</b> @37°C: 25% |
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