NEB, R0650S, FatI
FatI has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10201943.
Catalog Number: R0650S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to digest 1 µg of pUC19 DNA in 1 hour at 55°C in a total reaction volume of 50 μl. <b>Reaction Conditions</b> 1X NEBuffer™ r2.1 Incubate at 55°C 1X NEBuffer™ r2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Activity in NEBuffers</b> NEBuffer™ r1.1 | 10% NEBuffer™ r2.1: 100% NEBuffer™ r3.1: 50% rCutSmart™ Buffer: 50% <b>Diluent Compatibility</b> Diluent A <b>Storage Buffer</b> 10 mM Tris-HCl 50 mM NaCl 0.1 mM EDTA 1 mM DTT 50% Glycerol 200 µg/ml Recombinant Albumin pH 7.4 @ 25°C <b>Heat Inactivation</b> 80°C for 20 minutes <b>Methylation Sensitivity</b> dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive <b>Activity at Temperature</b> @37°C: 100% |
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