NEB, R0695S, PI-PspI
Catalog Number: R0695S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to cleave 1 µg of pAKR7 XmnI-linearized Control Plasmid in 1 hour at 65°C in a total reaction volume of 50 μl. <b>Reaction Conditions</b> 1X NEBuffer™ PI-PspI Supplement with Recombinant Albumin, Molecular Biology Grade Incubate at 65°C 1X NEBuffer™ PI-PspI 10 mM Tris-HCl 10 mM MgCl2 1 mM DTT 150 mM KCl (pH 9.2 @ 25°C) <b>Activity in NEBuffers</b> NEBuffer™ r1.1 | 10% NEBuffer™ r2.1: 10% NEBuffer™ r3.1: 10% rCutSmart™ Buffer: 10% <b>Diluent Compatibility</b> Diluent B <b>Storage Buffer</b> 10 mM Tris-HCl 300 mM NaCl 1 mM DTT 0.1 mM EDTA 500 µg/ml BSA 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> No <b>Activity at Temperature</b> @37°C: 10% |
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