NEB, R0696S, PI-SceI
The large size of this product was discontinued on 12/31/2020. The small size will continue to be available.
Catalog Number: R0696S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to cleave 1 μg of pBSvdeX XmnI-linearized Control Plasmid in 3 hours at 37°C in a total reaction volume of 50 μl. <b>Reaction Conditions</b> 1X NEBuffer™ PI-SceI Supplement with Recombinant Albumin, Molecular Biology Grade Incubate at 37°C 1X NEBuffer™ PI-SceI 10 mM MgCl2 1 mM DTT 10 mM Tris-HCl 100 mM KCl (pH 8.6 @ 25°C) <b>Activity in NEBuffers</b> NEBuffer™ r1.1 | 10% NEBuffer™ r2.1: 10% NEBuffer™ r3.1: 10% rCutSmart™ Buffer: 10% <b>Diluent Compatibility</b> Diluent B <b>Storage Buffer</b> 10 mM Tris-HCl 1 mM DTT 0.1 mM EDTA 500 µg/ml BSA 50% Glycerol 300 mM NaCl pH 7.4 @ 25°C <b>Heat Inactivation</b> 65°C for 20 minutes |
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