NEB, R0706S, Nb.BsmI
Catalog Number: R0706S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to convert 1 µg of supercoiled plasmid pBR322 DNA to open circular form in 1 hour at 65°C in a total reaction volume of 50 μl. <b>Reaction Conditions</b> 1X NEBuffer™ r3.1 Incubate at 65°C 1X NEBuffer™ r3.1 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Activity in NEBuffers</b> NEBuffer™ r1.1 | <10% NEBuffer™ r2.1: 50% NEBuffer™ r3.1: 100% rCutSmart™ Buffer: 10% <b>Diluent Compatibility</b> Diluent A <b>Storage Buffer</b> 10 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 200 µg/ml BSA pH 7.4 @ 25°C <b>Heat Inactivation</b> 80°C for 20 minutes <b>Methylation Sensitivity</b> dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive |
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