NEB, R3101S, EcoRI-HF®
EcoRI-HF has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10211229.
Catalog Number: R3101S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl. <b>Reaction Conditions</b> 1X rCutSmart™ Buffer Incubate at 37°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Activity in NEBuffers</b> NEBuffer™ r1.1 | 10% NEBuffer™ r2.1: 100% NEBuffer™ r3.1: <10% rCutSmart™ Buffer: 100% <b>Diluent Compatibility</b> Diluent C <b>Storage Buffer</b> 10 mM KPO4 300 mM NaCl 1 mM DTT 0.1 mM EDTA 200 µg/ml Recombinant Albumin 50% Glycerol 0.15% Triton® X-100 pH 7 @ 25°C <b>Heat Inactivation</b> 65°C for 20 minutes <b>Methylation Sensitivity</b> dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by Some Combinations of Overlapping |
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