Phenomenex, 00B-4800-AN, Biozen Native RP-5 (50 x 2.1), LC Column 50 X 2.1 mm, EA

The Phenomenex bioZen Native RP-5 liquid chromatography column is a protein-focused reversed-phase separation device designed for suitable native or non-denaturing LC workflows. The analytical bed is 50 mm long with a 2.1 mm internal diameter. It is used with appropriately selected aqueous-organic and volatile-salt conditions for intact biomolecule characterization, including analysis of antibody-drug conjugate species in LC–high-resolution mass spectrometry workflows. This specialized protein column should not be treated as an ordinary small-molecule C18 phase or automatically operated under conventional acidic protein-denaturing reverse-phase conditions. Mobile-phase choice, gradient conditions, sample loading and the associated instrument influence preservation of molecular complexes and interpretation of conjugate distributions. The manufacturer specifies its particle description as proprietary rather than an ordinary numeric particle size in the exact product listing. Follow an application-specific native reversed-phase method rather than assuming compatibility with every protein or mass-spectrometry assay.