Phenomenex, 00D-4449-Y0, Luna 3 µm HILIC 200 Å, LC Column 100 x 3 mm, Ea
Catalog Number: 00D-4449-Y0
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| Carbon Load % | 5.7 % |
|---|---|
| Surface Area (sq.Meter/g) | 200* m/g |
| USP Classification | L20 / L3 |
| Pore Size | 200.0000 Å |
| Length (mm) | 100.0000 mm |
| Particle Size | 3.0000 µm |
| Internal Diameter | 3.0000 mm |
| Film Thickness | 0.0000 µm |
Answer:Hydrophilic interaction liquid chromatography, typically with an acetonitrile-rich mobile phase.
Answer:100 × 3.0 mm with 3 µm particles and 200 Å pores.
Answer:Retention is sensitive to water content and mobile-phase composition, so sufficient equilibration improves reproducibility.
The Phenomenex Luna HILIC column is a hydrophilic-interaction liquid chromatography column for compounds that show limited retention in conventional reversed-phase separations. The packed bed uses 3 µm particles with 200 Å pores in a 100 × 3.0 mm analytical format. HILIC methods commonly use acetonitrile-rich mobile phases containing a controlled aqueous component; water content and salt concentration influence analyte retention and peak behavior. The column is useful for developing analytical separations of polar metabolites, ionic small molecules and other hydrophilic compounds when the sample and mobile phase are compatible. Its listed isocratic operating pH range is 1.5–8, and the specified maximum backpressure is 345 bar. Allow sufficient equilibration after changing the water fraction or buffer composition, since HILIC retention can be especially sensitive to these variables. Keep sample solvent composition compatible with the starting mobile phase to avoid peak distortion. This is a HILIC analytical column, not a C18 reversed-phase cartridge.
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