Phenomenex, 00G-4167-E0, Jupiter 5 µm C4 300 Å, LC Column 250 x 4.6 mm, Ea

Phenomenex Jupiter® C4 is a wide-pore reversed-phase HPLC column intended for separation of peptides, proteins, and other relatively large biomolecules. This configuration uses 5 µm silica particles with 300 Å pores in a 4.6 × 250 mm analytical column. The short-chain C4 bonded phase provides less hydrophobic retention than an otherwise comparable C18 column, potentially improving elution of larger, more hydrophobic biological analytes. The wide-pore packing facilitates interaction between large molecules and the bonded stationary phase. Typical applications include peptide mapping, protein separation, method development for biopharmaceutical samples, and characterization of purified proteins. The 250 mm length provides chromatographic resolving power for workflows that can accommodate conventional analytical HPLC operating times and pressures.