Qiagen, 1022070, Luciferase GL2 siRNA (5 nmol)

During experimental setup using a reporter-gene construct, a reporter control siRNA can be used to determine optimal conditions. When using a reporter-gene construct, a reporter control siRNA transfected in every experiment will indicate if conditions become suboptimal. Cotransfection of a plasmid carrying a reporter gene and a reporter control siRNA is a commonly used control in high-throughput RNAi.
AllStars Reporter Controls can be routinely transfected in experiments using reporter-gene constructs (such as plasmids carrying the reporter gene) to ensure optimal conditions are maintained. The AllStars Reporter Control should always result in high reporter-gene knockdown. If high knockdown is not achieved, this indicates a problem with the experimental setup.