Description
The Investigator Casework GO! Kit can be used to perform lysis of forensic samples on a variety of substrates, including sexual assault swabs, pieces of fabric, paper, cigarette butts, chewing gum and other sample types. The generated lysates are compatible with all Investigator Quantiplex and Investigator STR QS Systems. The Investigator Casework GO! Kit meets ISO 18385 requirements.
The Investigator Casework GO! Kit is used for the pretreatment of forensic samples in a direct amplification workflow. The kit permits sample lysis without DNA purification and the lysate is compatible with several downstream options, including the Investigator Quantiplex Pro, Investigator Quantiplex Pro FLX and Quantiplex Pro RGQ or the Investigator 24plex QS, 26plex QS, ESSplex SE QS or IDplex Plus Kits. The Investigator Casework GO! Kit can be used for direct amplification of casework samples and thus enables an accelerated workflow in casework analysis. It allows the rapid processing of swabs from casework samples, including cuttings of sexual assault swabs, or other materials, without the need for further purification. The lysate can be used directly for quantification with all Investigator Quantiplex assays or for DNA profiling using all Investigator STR assays (unless data from the quantification indicates presence of possible PCR inhibitors). In sexual assault screening analyses, the Investigator Casework GO! workflow can facilitate sample processing decisions by male-quantification and Auto/Y ratio.
Add 100–400 µL of Casework GO! Master Mix to the sample in a tube and incubate at 60°C for 25 minutes followed by an additional 5 minutes at 80°C. Quantify 2 µL of this lysate using the Quantiplex Pro kit, or up to 18 µL using the Quantiplex Pro FLX kit and use up to 15 µL of the lysate for amplification using the Investigator STR QS Kit.
