Description
The QIAamp Fast DNA Stool Mini Kit enables rapid purification of high-quality genomic DNA (human and bacterial) from fresh or frozen stool samples. The novel InhibitEX Buffer replaces cumbersome inhibitor removal tablets to efficiently remove PCR inhibitors commonly present in stool samples. Fewer protocol steps streamlines your workflow and reduces hands-on time during extraction. The QIAamp Fast DNA Stool Mini Kit may be used with a microcentrifuge and may be automated on the QIAcube Connect.
Superior yields for best downstream sensitivity The improved protocol of the QIAamp Fast DNA Stool Mini Kit ensures highest yields from stool samples in less time than commonly used methods. Furthermore, hazardous organic chemicals are not used, improving laboratory and personal safety. Typical yields of DNA obtained with the QIAamp Fast DNA Stool Mini Kit are 5–50 µg, which is consistently higher than comparable purification kits from alternative suppliers (see figure Superior yields compared to similar purification kits). DNA purified with the QIAamp Fast DNA Stool Mini Kit gives highly sensitive results in downstream quantitative real-time PCR, with consistently lower C T values than obtained with solutions from other suppliers (see figure Higher sensitivity in qPCR). Effective removal of inhibitors Stool samples are rich in PCR inhibitors like complex polysaccharides, bile salts, lipids, and urate. In best cases, these inhibitors make targets difficult to amplify. In worst cases, their presence can entirely suppress PCR signals. Therefore, a method to completely remove such inhibitors is critical for any stool sample analysis. The novel InhibitEx Buffer of the QIAamp Fast DNA Stool Mini Kit efficiently depletes PCR inhibitors from samples, enabling reliable detection of DNA (see figure Efficient depletion of PCR inhibitors).
DNA binds specifically to the QIAamp silica-gel membrane while contaminants pass through. No phenol-chloroform extraction is required. PCR inhibitors are separated from DNA by the specially formulated chemistry of the InhibitEX Buffer. Highly pure DNA ready for direct use in downstream applications is purified in as little as 25 minutes. QIAamp sample preparation technology is fully licensed.
The QIAamp Fast DNA Stool Mini Kit simplifies isolation of DNA from stool with a fast spin-column procedure (see flowchart Streamlined procedure of the QIAamp Fast DNA Stool Mini Kit) and streamlined protocols, reducing the total time needed from sample to isolated DNA to as little as 25 minutes. The unique formulation of the InhibitEX Buffer efficiently separates PCR inhibitors from DNA and, because of its liquid format, is more convenient than tablets or powder. Manual sample handling is streamlined because separate addition of inhibitor removal reagents is no longer required and experiments can be scaled more easily. The QIAamp Fast DNA Stool Mini Kit can be automated on instruments like the QIAcube. Rapid protocols are provided to efficiently isolate human DNA as well as microbial DNA for pathogen detection. Shortly, stool is suspended in InhibitEX Buffer, which separates inhibitors from DNA. Following a quick lysis, DNA binds the QIAamp silica membrane. Any remaining inhibitors and contaminants are removed by washing steps and pure, intact DNA is then eluted from the column.
