Qiagen, 32915, N-Terminus pQE Vector Set

This set provides 5 vectors (pQE-9, pQE-30, pQE-31, pQE-32, and pQE-40) for expression of N-terminally His-tagged proteins. pQE-30, pQE-31, and pQE-32 provide the multiple cloning site (MCS) in all three reading frames while pQE-9 has an alternative, shorter multiple cloning site. pQE-40 is designed for expression of DHFR-fusion proteins and is recommended for expression of poorly expressed proteins or short peptides, which are often prone to proteolysis, as DHFR enhances both stability and antigenicity. Since DHFR itself displays little immunogenicity in mouse and rat, DHFR-fusion proteins are ideal for epitope screening.
Elements present in QIA express pQE vectors Element Description Optimized promoter/operator element Consists of the phage T5 promoter and two lac operator sequences, which increase the probability of lac repressor binding and ensure efficient repression of the powerful T5 promoter Synthetic ribosomal binding site RBSII For efficient translation 6xHis-tag coding sequence Either 5' or 3' to the polylinker cloning region Translational stop codons In all reading frames for convenient preparation of expression constructs Two strong transcriptional terminators t 0 from phage lambda, and T1 from rrnB operon of E. coli, to prevent read-through transcription and ensure stability of the expression construct ColE1 origin of replication From pBR322 Beta-lactamase gene (bla) Confers ampicillin resistance
Inserts encoding proteins of interest are cloned into appropriate constructs (For detailed information see the QIAexpressionist Handbook) and transformed into a suitable E. coli strain for expression. Expression is induced by addition of IPTG. Vector pQE-TriSystem constructs can be transformed into E. coli, used as a shuttle vector for recombinant protein expression in insect cells, or transfected into mammalian cells.