Description
RT 2 SYBR Green FAST Mastermixes are specially formulated for use with the 100-ring disc format of the RT 2 Profiler and RT 2 lncRNA PCR arrays on the Rotor-Gene Q. This mastermix is suited for use with self-designed qPCR assays on other types of real-time PCR instruments with fast cycling conditions. RT 2 SYBR Green FAST Mastermixes are available with ROX, fluorescein, or without reference dyes.
RT² SYBR Green FAST Mastermixes contain all of the optimized reagents and buffers needed for SYBR Green based real-time PCR with RT 2 qPCR Primer Assays and RT 2 Profiler PCR Arrays on all real-time PCR instruments. The high-performance real-time PCR enabled by RT² SYBR Green FAST Mastermixes demonstrates high amplification efficiencies (see figure "High amplification efficiency over a wide dynamic range") and high levels of sensitivity and specificity (see figure "Tighter control of polymerase activity yields greater specificity"). In addition, dissociation curves obtained using RT 2 SYBR Green FAST Mastermixes have lower background and a sharper shape compared with dissociation curves generated by regular cycling conditions (see figure "Sharper dissociation curves with RT 2 SYBR Green Fast Mastermix").
RT 2 SYBR Green FAST Mastermixes contain real-time PCR buffer, a high-performance, HotStart DNA Taq polymerase, nucleotides, and SYBR Green dye. Some mastermixes contain either fluorescein or ROX dye for optimization of the instrument optics. The chemically-modified and tightly controlled HotStart enzyme uniquely provides accurate SYBR Green results by preventing the amplification of primer–dimers and other nonspecific products. RT 2 SYBR Green FAST Mastermixes are optimized to use a 10-second denaturation step and 30-second annealing/extension step PCR protocol, without compromising specificity or sensitivity. Mastermixes without ROX or fluorescein RT 2 SYBR Green FAST Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation not requiring a reference dye, including Bio-Rad models CFX96, CFX384; Bio-Rad/MJ Research Chromo4, DNA Engine Opticon, DNA Engine Opticon 2; Roche LightCycler 480 (96-well and 384-well); Eppendorf Mastercycler ep realplex without ROX filter set; and Cepheid SmartCycler. Mastermixes with fluorescein RT 2 SYBR Green Fluor FAST Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation that uses fluorescein as a reference dye, including the Bio-Rad models iCycler, iQ5, MyiQ, and MyiQ2. Mastermixes with ROX RT 2 SYBR Green ROX FAST Mastermix is highly suited for real-time PCR applications using SYBR Green-based detection on instrumentation that uses ROX as a reference dye, including QIAGEN’s Rotor-Gene Q; Applied Biosystems models 5700, 7000, 7300, 7500 (Standard and Fast), 7700, 7900HT (Standard and Fast 96-well block, 384-well block), StepOnePlus, ViiA 7 (Standard and Fast 96-well block, 384-well block); Eppendorf Mastercycler ep realplex with or without ROX filter set; Stratagene models Mx3000P, Mx3005P, Mx4000; and Takara TP-800.
