Qiagen, 338322, Custom qPCR LNA Assay (2 primers)
Primer pair designed by highly skilled experts using optimized internal design tools.
Catalog Number: 338322
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| Verified Reactivity | Human |
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| Reported Reactivity | African Green, Baboon, Cynomolgus, Rhesus |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). |
| Preparation | The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions. |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Application | FC - Quality tested |
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| Recommended Usage |
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| Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. |
| Excitation Laser | Violet Laser (405 nm) |
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| Application Notes | Additional reported applications include: block NK cell-mediated lysis. |
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| Application References(PubMed link indicates BioLegend citation) | Fuchs A, et al. 2004. J. Immunol. 172:3994. |
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| Product Citations | Blache U, et al. 2021. Front Immunol. 12:658314. PubMed |
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| RRID | AB_2721559 (BioLegend Cat. No. 338321) AB_2721560 (BioLegend Cat. No. 338322) |
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| Structure | Ig domain containing adhesion molecule |
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| Distribution | Peripheral blood T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells |
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| Function | Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell |
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| Interaction | Interacts with LFA-1. |
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| Ligand/Receptor | PVR (CD155) and Nectin-2 (CD112) |
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| Cell Type | B cells, Macrophages, Megakaryocytes, Monocytes, NK cells, Platelets, T cells |
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| Biology Area | Immunology |
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| Molecular Family | CD Molecules |
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| Antigen References | 1. Shibuya A et al. 1996. Immunity 4:573. 2. Tahara-Hanaoka S et al. 2004. Int. Immunol. 16:533. 3. Pende D et al. 2005. Mol. Immunol. 42:463. |
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| Gene ID | 10666 |
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| UniProt | View information about CD226 on UniProt.org |
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| Clone | 11A8 |
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| Regulatory Status | RUO |
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| Other Names | DNAM-1, PTA1, TLiSA1 |
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| Isotype | Mouse IgG1, κ |
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