Qiagen, 37502, Qproteome Cell Compartment Kit

Eukaryotic cells are complex, well-ordered, and highly structured systems. The Cell Compartment Kit is designed for fast and easy subcellular fractionation of intact eukaryotic cells and tissue. By sequential addition of different extraction buffers to a cell pellet, proteins in the different cellular compartments can be selectively isolated.
Use of the Qproteome Cell Compartment Kit allows simple and effective cell compartment-specific localization of marker proteins from isolated cells (see figure "Specific Separation of Marker Proteins "and to "Localization of Proteins in Cells Under Different Growth Conditions") or from tissue samples (see figure "Localization of Proteins in Tissue Samples").
The kit contains 4 Extraction Buffers which enable the sequential isolation of proteins associated with the cytosol, membranes, nucleus, and cytoskeleton from cell lysates or tissues.
The 4 Extraction Buffers are added sequentially to a cell pellet and the respective fractions are isolated by centrifugation (see flowchart). In an alternative protocol for tissues, samples are simultaneously homogenized and disrupted using the TissueRuptor before being processed as for the cell pellet.