Description
Investigator 24plex Kits are for human identification, allowing multiplex amplification of the CODIS (Combined DNA Index System) core loci, the ESS (European Standard Set) markers, plus SE33, DYS391 and Amelogenin. The kits include an innovative Quality Sensor, enabling invaluable additional data to be generated for quality control and performance tests. Furthermore, the Investigator 24plex GO! Kit enables direct amplification from reference samples without prior DNA extraction. Investigator 24plex Kits meet ISO 18385 requirements. Want to try the Investigator 24plex GO! Kit for the first time? Request a quote for a trial kit. Want to try the Investigator 24plex QS Kit for the first time? Request a quote for a trial kit.
Investigator 24plex Kits use fast-cycling technology to provide reliable results in approximately 45–60 minutes. The Fast Reaction Mix 2.0 is based on proven QIAGEN multiplex PCR technology. It has been further optimized for ease of handling and increased speed and sensitivity with the Investigator 24plex QS Kit and for direct amplification without prior DNA extraction with the Investigator 24plex GO! Kit. Assay performance tested in the presence of increasing concentrations of inhibitors shows superior inhibitor resistance. This enables reliable, high-quality results from casework samples, but also from direct amplification of typical reference samples such as blood or epithelial cells on FTA paper. Investigator 24plex Kits were validated according to ENFSI and SWGDAM guidelines. The recommended amount of DNA under standard conditions for the Investigator 24plex QS Kit is 0.5 ng. Internal validations demonstrated robust and balanced results with 0.2 to 2 ng DNA, and reliable results with <0.1 ng DNA.
Investigator 24plex Kits use QIAGEN's fast-cycling PCR technology for rapid and reliable co-amplification of the CODIS and ESS markers, plus SE33, DYS391 and Amelogenin. Carefully designed primers reduce overlap of loci alleles, thereby minimizing the likelihood of data misinterpretation. The innovative Quality Sensors provide additional information on sample quality and PCR success beyond the profile of the sample. With two Quality Sensors being amplified as a positive control, it is possible to determine: If amplification was successful If DNA was present in the sample If the sample was degraded If inhibitors in the sample prevented successful PCR This additional information will greatly enhance your data analysis and will help you to make crucial decisions with respect to further sample processing. The Quality Sensors will help you reduce unnecessary re-runs, saving you time and money. The two gender markers, Amelogenin and DYS391, have been optimized to produce small fragment sizes, making the gender typing extremely robust. Even for populations with Amelogenin-null mutations, this means no more failures with the essential gender typing, even with degraded or inhibited samples.
The 22 STR markers, the gender-specific Amelogenin and the Quality Sensor are amplified simultaneously in a single PCR and labeled with one of the dyes listed in the table "Investigator 24plex Kit dyes and corresponding markers". The amplified loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as Applied Biosystems GeneMapper ID-X. See the table "Technical specifications "for more information. Investigator 24plex Kit dyes and corresponding markers Dye Marker 6-FAM Amelogenin TH01 D3S1358 vWA D21S11 BTG TPOX DYS391 D1S1656 D12S391 SE33 BTY D10S1248 D22S1045 D19S433 D8S1179 D2S1338 BTR2 D2S441 D18S51 FGA BTP QS1 D16S539 CSF1PO D13S317 D5S818 D7S820 QS2
