Description
QIAamp DNA Kits provide silica-membrane-based nucleic acid purification from tissues, swabs, CSF, blood, body fluids or washed cells from urine. In addition, genomic and mitochondrial DNA can be purified from small amounts of fresh or frozen blood, tissue and dried blood spots. Mechanical homogenization is not required as the tissues are lysed enzymatically. DNA purification from 1–12 samples can be automated on the QIAcube Connect using the dedicated QIAamp DNA Mini QIAcube Kit. Purification of DNA using the QIAamp DNA Micro Kit and QIAamp DNA Mini Kit is also automatable on the QIAcube Connect. QIAamp DNA Mini standard protocols can also be executed using the TRACKMAN Connected system.
The QIAamp DNA Micro Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 µL. DNA purified using the QIAamp DNA Micro Kit is free of proteins, nucleases and other impurities, and is suitable for use in sensitive downstream applications, such as real-time PCR (see figure "Efficient purification of DNA from small sample sizes") and laser microdissection (LMD) PCR (see figure "Laser microdissection PCR"). Purified DNA may also be used in short-tandem repeat (STR) genotyping, single-nucleotide polymorphism (SNP) genotyping or pharmacogenomic research. The QIAamp DNA Mini Kit simplifies DNA isolation from tissue samples with fast spin-column or vacuum procedures, yielding DNA sized up to 50 kb (see figure "Purification of up to 50 kb genomic DNA"). DNA of this length denatures completely and has the highest amplification efficiency. Yields of nucleic acids or DNA depend on the starting material (see table “Typical yields with the QIAamp DNA Mini Kit”). DNA purified using the QIAamp DNA Mini Kit can be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, Southern blotting, SNP and STR genotyping and pharmacogenomic research. Sample Amount Total nucleic acid yields (µg)* DNA yields (µg)† Blood 200 µL 4–12 4–12 Buffy coat 200 µL 25–50 25–50 Cells 10 6 20–30 15–20 Liver 25 mg 60–115 10–30 Brain 25 mg 35–60 15–30 Lung 25 mg 25–45 5–10 Heart 25 mg 15–40 5–10 Kidney 25 mg 40–85 15–30 Spleen 10 mg 25–45 5–30 The QIAamp DNA Mini QIAcube Kit yields DNA sized up to 50 kb: DNA of this length denatures completely and has the highest amplification efficiency. The purified DNA may be used in a number of applications, including: Viral research Bacterial research Fungal research Cancer research Human genetic testing research Paternity testing Forensic analysis The QIAamp DNA Mini QIAcube Kit simplifies DNA isolation from tissue samples with fast spin-column procedures. The kit includes rotor adapters that are preloaded with QIAamp spin columns and elution tubes, delivering greater convenience and time savings (see figure "Significant time savings").
During the QIAamp DNA purification procedure, DNA binds specifically to the QIAamp MinElute or QIAamp silica-gel membrane while contaminants pass through. No phenol-chloroform extraction is required. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure DNA to be eluted in either water or a buffer provided with the kit. QIAamp DNA technology yields genomic, mitochondrial, bacterial, parasite or viral DNA from human tissue samples ready to use in PCR and blotting procedures. QIAamp sample preparation technology is fully licensed, allowing QIAamp purified nucleic acids to be used in any molecular assay or other downstream application without risk of patent infringement.
Optimized buffers and enzymes in the QIAamp DNA Mini Kit lyse samples, stabilize nucleic acids and enhance selective DNA adsorption to the QIAamp membrane. Alcohol is added and lysates loaded onto the QIAamp spin column. Wash buffers are used to remove impurities and pure, ready-to-use DNA is then eluted in water or low-salt buffer. No mechanical homogenization is necessary as the tissues are lysed enzymatically. The convenient spin-column procedure means that hands-on preparation time is only 20 minutes (lysis times differ according to the sample source). Samples can be processed using either a microcentrifuge or, if blood or other body fluids are being processed, using the QIAvac 24 Plus. In addition, the rigorous lysis procedure employed makes the QIAamp DNA Mini Kit ideal for purification of genomic DNA from bacteria or parasites. To further reduce hands-on time, genomic DNA purification may be automated on the QIAcube. The QIAamp DNA Accessory Set A provides the additional buffers and reagents needed for isolation of genomic, mitochondrial, bacterial, parasite or viral DNA using the QIAcube with 12 x QIAamp DNA Mini Kits (50). Vacuum processing Blood or other body fluids can be processed by vacuum, instead of centrifugation, for greater speed and convenience in DNA purification. QIAamp Mini spin columns are accommodated on the QIAvac 24 Plus manifold using VacValves and VacConnectors. VacValves should be used if sample flow rates differ significantly, to ensure consistent vacuum. Disposable VacConnectors are used to avoid any cross-contamination. Use of VacConnectors also allows QIAamp spin procedures to be performed on QIAvac 6S with QIAvac Luer Adapters. Automated DNA isolation on QIAcube Connect The dedicated QIAamp DNA Mini QIAcube Kit is designed with preloaded spin columns and elution tubes in rotor adapters for QIAcube instruments, eliminating the risk of errors due to incorrect loading of rotor adapters. The dedicated kit is tailored to QIAcube requirements, reducing waste. A simple protocol (lyse, bind, wash and elute) and an automated protocol on QIAcube Connect requires minimal user interaction. Award-winning QIAcube Connect uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into laboratory workflows. All steps in the purification procedure are fully automated — up to 12 samples can be processed per run. QIAcube Connect and the dedicated QIAamp DNA Mini QIAcube Kit provide a winning combination for fast, easy and convenient DNA purification.
