Description
Recombinant Albumin, AOF, is an animal-origin-free (AOF) protein designed to deliver high consistency, purity and safety for demanding molecular biology workflows. Albumin acts as a stabilizer for enzymes and nucleic acids and can bind PCR inhibitors, helping ensure consistent amplification even from challenging samples. Its high purity, thermostability and animal-origin-free composition make it a reliable choice for demanding amplification workflows, especially where consistency and safety are priorities. QIAGEN Recombinant Albumin, unlike animal-derived BSA, eliminates the risks of contamination, variability and regulatory hurdles, ensuring reliable results in sensitive applications. Recombinant Albumin, AOF, is a genetically engineered protein with increased thermal stability to reduce aggregation and ensure reliable, high-yield amplification.
QIAGEN Recombinant Albumin, AOF, has improved thermal stability that minimizes protein aggregation, enhancing amplification efficiency and yield (see Figure 1, Increased thermostability). Recombinant Albumin exhibits low aggregate content, contributing to improved reaction performance, better stability and enhanced reproducibility in molecular biology workflows. (See figure 2, Ultra-low aggregate content). Recombinant Albumin is stable for at most 10 freeze–thaw cycles (from −20°C to room temperature), but the optimal storage temperature is 2–8°C. (See Figure 3, Resistance to freeze-thaw cycles). Recombinant Albumin, AOF, mitigates the effects of common PCR inhibitors, maintaining assay sensitivity and reproducibility (See Figure 4, Recombinant Albumin reduces qPCR inhibition).
Albumins function as stabilizers, carriers and inhibitor-binding proteins in molecular biology reactions. By binding inhibitory molecules present in environmental samples, such as humic acid, hematin, melanin and others, they enhance stability and reproducibility in PCR, qPCR and related applications. Recombinant Albumin, AOF, is a 66.4 kDa human serum albumin, overexpressed in yeast to deliver high purity and performance. The yeast expression system ensures lot-to-lot consistency, enabling reproducible results and seamless scalability. Features of Recombinant Albumin, AOF. Parameter Value Source Yeast (recombinant expression) Concentration 100 g/L (10%) Purity ≥95% Endonuclease activity None detected Exonuclease activity None detected Protease activity None detected RNase contamination None detected Host DNA contamination Not detectable Endotoxin 5 mM) in the final albumin solution. High concentrations of DTT are known to cause precipitation of albumin.
