Description
The ipsogen MPL W515L/K Muta Screen Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The ipsogen MPL W515L/K Muta Screen Kit provides reagents optimized for reliable and sensitive detection and qualitative analysis of the MPL W515L and MPL W515K mutations in human genomic DNA.
To ensure accuracy, the ipsogen MPL W515L/K Muta Screen Kit has been optimized to detect the MPL W515L and W515K mutations and uses DNA positive and negative controls for qualitative analysis (see figure "Representative allelic discrimination"). The ipsogen MPL W515L/K Muta Screen Kit provides DNA cut-off samples (COS) corresponding to 1.5% of MPL W515K and 1.5% of MPL W515L, ensuring accurate qualitative analysis.
The ipsogen MPL W515L/K Muta Screen Kit is a ready-to-use kit for the detection and qualitative analysis of the MPL W515L and W515K mutations independently in human genomic DNA (see figure "MPL mutations"). The kit provides reliable and reproducible results. The ipsogen MPL W515L/K Muta Screen Kit provides DNA cut-off samples (COS) for MPL W515L and MPL W515K mutations, DNA positive controls (PC), and a negative control (NC) for MPL W515L and MPL W515K. Use of the cut-off samples and controls enables accurate qualitative analysis of genotyping results.
The first step is extraction and determination of quality and quantity of genomic DNA. The second step is the amplification of DNA by real-time PCR. Using the ipsogen MPL W515L/K Muta Screen Kit provides reliable detection and qualitative analysis of the MPL W515L and W515K mutations independently. Simply start the reaction using the optimized protocols described in the kit handbook.
