Revvity, 6096731, ATPlite 1glow-F, 100 ml

ATP is a marker for cell viability due to its presence in all metabolically active cells. ATP concentration declines rapidly when cells undergo necrosis or apoptosis, and so monitoring ATP is a good indicator of cytotoxic, cytostatic and proliferation effects. Our ATPlite 1glow F, in a ready-to-use liquid format, is an advanced luminescence assay that detects ATP in mammalian cells using firefly luciferase. As ATP levels correlate with cell viability, this assay is a powerful tool for assessing cytotoxicity and proliferation. Engineered for signal stability, this luminescence based technology provides a broad dynamic range and can reduce interference from colored compounds that may affect absorbance-based viability assays. The ATPlite 1glow F reagent utilizes a homogenous "add-mix-read" format, eliminating wash and separation steps to simplify workflows in high-throughput applications. It features enhanced signal stability with a long signal half-life, offering greater flexibility for batch processing. This single reagent measures ATP changes in both 2D and 3D cell culture.